Liquid chromatography (LC) is the standard method used for purifying products in the discovery stages, as it is cost-effective, relatively simple to perform, and reliable. However, obtaining high purity and yield using this approach often comes at the cost of throughput, as purification runs must be lengthened to achieve greater separation between components. This article explores how users can enhance fraction collection efficiency and streamline the purification process by using the latest advances.

In this article, we hear from an industry leader on their experience using centrifugal partition chromatography (CPC) in the pharmaceutical industry, and how it's a method well-suited for producing highly active molecules that meet safety and performance requirements.

While more than 220 viruses are known to infect humans, clinically approved antiviral drugs are available for only a dozen of these viruses. CPC is an ideal solution for natural compound investigations. Gilson, in collaboration with Brazilian researchers, recently performed preliminary experiments demonstrating the antiviral activity of compounds that were fractionated from a plant crude extract using CPC. Read more about the study here.

Using the right systems can improve sample integrity in your sample separation workflows, helping labs to have greater confidence in their results. Here we outline what to look for in your sample separation instruments to remove risks of common threats to sample integrity.

Fractionation, or the separation of a mixture of molecules into different parts or “fractions,” helps scientists understand and/or use a complex solution. Collecting and isolating individual fractions from a mixture can enable many downstream applications as specific molecules can be separated based on physical and chemical properties. In this article, we explore the basics of fraction collection, including applications, parameters, and available solutions for your lab.

In high performance liquid chromatography (HPLC), the selection of an appropriate mode of detection is vital to the success of any separation. To this end, there are numerous choices one might consider when configuring a new HPLC setup. Mass spectrometry (MS) is an alternative method of detection and identification that is much more robust than UV. Here, we describe how the VERITY 1920 Mass Spectrometer allows for MS detection capabilities at an affordable price for HPLC fraction collection.

In this technical note, we highlight how our software and products help you add extra dimensions to your fraction collection possibilities allowing you to set conditions according to specific requirements for synthetic compound purification.

Centrifugal Partition Chromatography (CPC), also known as Counter Current Chromatography (CCC) is a preparative, pilot and industrial scale liquid purification technique that does not require traditional solid supports.

In this video, you will learn about the benefits of Centrifugal Partition Chromatography (CPC) as it relates to other forms of traditional sample separation. Without solid support, liquid-liquid chromatography allows for repeat injections of samples with 100% recovery of the materials--and you'll never need to replace any silica columns. CPC columns tackle tough separations of complex samples in a single step without prior purification, making it an ideal choice for natural product isolation, protein purification, and more.

There are many different chromatography techniques, but which is right for your experiment? This video will give an overview of the main differences between analytical and preparative chromatography and help guide you to the technique that is best for your lab's purification research.